{"id":567,"date":"2023-07-22T14:18:20","date_gmt":"2023-07-22T14:18:20","guid":{"rendered":"https:\/\/chemuza.org\/tr\/bromofenol-mavisi-c19h10br4o5s\/"},"modified":"2023-07-22T14:18:20","modified_gmt":"2023-07-22T14:18:20","slug":"bromofenol-mavisi-c19h10br4o5s","status":"publish","type":"post","link":"https:\/\/chemuza.org\/tr\/bromofenol-mavisi-c19h10br4o5s\/","title":{"rendered":"Bromofenol mavisi \u2013 c19h10br4o5s, 115-39-9"},"content":{"rendered":"

Bromofenol mavisi (BPB), pH 3,0’\u0131n alt\u0131nda sar\u0131ya ve pH 4,6’n\u0131n \u00fczerinde maviye d\u00f6nen bir pH g\u00f6stergesidir. Molek\u00fcler biyolojide DNA elektroforezi i\u00e7in izleme boyas\u0131 olarak yayg\u0131n olarak kullan\u0131l\u0131r.<\/p>\n

\n\n\n\n\n\n\n\n
IUPAC Ad\u0131<\/td>\n 3′,3″,5′,5″-tetrabromofenol s\u00fclfoneftalein<\/td>\n<\/tr>\n
Molek\u00fcler form\u00fcl<\/td>\n C19H10Br4O5S<\/td>\n<\/tr>\n
CAS numaras\u0131<\/td>\n 115-39-9<\/td>\n<\/tr>\n
E\u015f anlaml\u0131<\/td>\n 3′,3′,5′,5′-tetrabromofenol s\u00fclfonftalein, TBB, bromofenol mavisi sodyum tuzu ve bromofenol mavisi serbest asit<\/td>\n<\/tr>\n
InChI<\/td>\n InChI=1S\/C19H10Br4O5S\/c20-13-5-1-12(2-6-13)19(11-24)27(25,26)17-9-15(29(21,22)23)7- 3-14(17)18(10-16(19)30(25,26)28)31(4-8-32(25,26)27)33(34)35\/h1-10,24H,11H2, (H,21,22,23)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n

Bromofenol Mavi Molar K\u00fctle<\/p>\n

BPB’nin molar k\u00fctlesi 669,96 g\/mol’d\u00fcr. Molar k\u00fctle, bir maddenin bir mol\u00fcn\u00fcn mol ba\u015f\u0131na gram cinsinden ifade edilen k\u00fctlesini ifade eder. Bir bile\u015fi\u011fin molar k\u00fctlesi, onu olu\u015fturan atomlar\u0131n her birinin atomik k\u00fctleleri toplanarak hesaplanabilir. BPB, karbon, hidrojen, oksijen, k\u00fck\u00fcrt ve brom atomlar\u0131 i\u00e7eren karma\u015f\u0131k bir organik bile\u015fiktir ve molar k\u00fctlesi, bu elementlerin her birinin atomik k\u00fctlelerinin bile\u015fi\u011fe eklenmesiyle hesaplan\u0131r.<\/p>\n

Bromofenol mavisinin kaynama noktas\u0131<\/h6>\n

BPB’nin kaynama noktas\u0131 yakla\u015f\u0131k 600\u00b0C’dir. Bir maddenin kaynama noktas\u0131, maddenin buhar bas\u0131nc\u0131n\u0131n atmosfer bas\u0131nc\u0131na e\u015fit oldu\u011fu s\u0131cakl\u0131kt\u0131r. Bu s\u0131cakl\u0131kta s\u0131v\u0131 gaz haline ge\u00e7er. BPB, b\u00fcy\u00fck boyutu ve karma\u015f\u0131k yap\u0131s\u0131 nedeniyle y\u00fcksek kaynama noktas\u0131na sahiptir ve molek\u00fclleri bir arada tutan molek\u00fcller aras\u0131 kuvvetleri par\u00e7alamak i\u00e7in \u00f6nemli miktarda enerji gerektirir.<\/p>\n

Bromofenol mavisi Erime noktas\u0131<\/p>\n

BPB’nin erime noktas\u0131 yakla\u015f\u0131k 218\u00b0C’dir. Bir maddenin erime noktas\u0131, maddenin kat\u0131 formunun s\u0131v\u0131 formuna d\u00f6n\u00fc\u015ft\u00fc\u011f\u00fc s\u0131cakl\u0131kt\u0131r. BPB’nin erime noktas\u0131, kristal kafeste mevcut olan g\u00fc\u00e7l\u00fc molek\u00fcller aras\u0131 kuvvetler nedeniyle nispeten y\u00fcksektir.<\/p>\n

Bromofenol mavisi Yo\u011funluk g\/ml<\/h6>\n

BPB’nin yo\u011funlu\u011fu yakla\u015f\u0131k 1,8 g\/ml’dir. Yo\u011funluk, bir maddenin birim hacmi ba\u015f\u0131na k\u00fctle miktar\u0131n\u0131 tan\u0131mlayan fiziksel bir \u00f6zelliktir. BPB’nin yo\u011funlu\u011fu karma\u015f\u0131k yap\u0131s\u0131ndan dolay\u0131 nispeten y\u00fcksektir ve birim hacim ba\u015f\u0131na y\u00fcksek k\u00fctleye neden olur.<\/p>\n

Bromofenol Mavisi Molek\u00fcl A\u011f\u0131rl\u0131\u011f\u0131<\/p>\n

BPB’nin molek\u00fcler a\u011f\u0131rl\u0131\u011f\u0131 669,96 g\/mol’d\u00fcr. Molek\u00fcl a\u011f\u0131rl\u0131\u011f\u0131, bir molek\u00fcldeki t\u00fcm atomlar\u0131n atom a\u011f\u0131rl\u0131klar\u0131n\u0131n toplam\u0131d\u0131r. Bir maddenin molek\u00fcler a\u011f\u0131rl\u0131\u011f\u0131, belirli bir \u00e7\u00f6zelti konsantrasyonunu haz\u0131rlamak i\u00e7in gereken madde miktar\u0131n\u0131n belirlenmesi gibi \u00e7e\u015fitli hesaplamalarda kullan\u0131l\u0131r. <\/p>\n

\n
\"Bromofenol<\/figure>\n<\/div>\n

Bromofenol mavisinin yap\u0131s\u0131<\/p>\n

BPB, d\u00f6rt brom atomu, iki fenol grubu ve bir s\u00fclfonat grubu i\u00e7eren bir s\u00fclfoneftalein yap\u0131s\u0131na sahiptir. Molek\u00fcl\u00fcn iki fenol grubuna ve bir s\u00fclfonat grubuna ba\u011fl\u0131 merkezi bir karbon atomu vard\u0131r. Molek\u00fcl ayr\u0131ca fenol gruplar\u0131n\u0131n her birine ba\u011fl\u0131 iki brom atomu i\u00e7erir. S\u00fclfonat grubu negatif y\u00fckl\u00fcd\u00fcr ve bu da BPB’yi suda \u00e7\u00f6z\u00fcn\u00fcr bir boya haline getirir.<\/p>\n

Bromofenol Mavisi Form\u00fcl\u00fc<\/h6>\n

BPB’nin kimyasal form\u00fcl\u00fc C19H10Br4O5S’dir. Form\u00fcl, mevcut atomlar\u0131n say\u0131s\u0131 ve t\u00fcr\u00fc a\u00e7\u0131s\u0131ndan molek\u00fcl\u00fcn bile\u015fimini temsil eder. Form\u00fcl, BPB’nin 19 karbon atomu, 10 hidrojen atomu, d\u00f6rt brom atomu, be\u015f oksijen atomu ve bir k\u00fck\u00fcrt atomu i\u00e7erdi\u011fini g\u00f6sterir.<\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
D\u0131\u015f g\u00f6r\u00fcn\u00fc\u015f<\/td>\n Koyu mavi kristaller veya toz<\/td>\n<\/tr>\n
Spesifik yer \u00e7ekimi<\/td>\n 1,82 – 1,85 g\/cm\u00b3<\/td>\n<\/tr>\n
Renk<\/td>\n Koyu mavi<\/td>\n<\/tr>\n
Koku<\/td>\n Kokusuz<\/td>\n<\/tr>\n
Molar k\u00fctle<\/td>\n 669,96 gr\/mol<\/td>\n<\/tr>\n
Yo\u011funluk<\/td>\n 1,8 g\/cm\u00b3<\/td>\n<\/tr>\n
F\u00fczyon noktas\u0131<\/td>\n ~218\u00b0C<\/td>\n<\/tr>\n
Kaynama noktas\u0131<\/td>\n ~600\u00b0C<\/td>\n<\/tr>\n
Fla\u015f noktas\u0131<\/td>\n Uygulanamaz<\/td>\n<\/tr>\n
sudaki \u00e7\u00f6z\u00fcn\u00fcrl\u00fck<\/td>\n Suda \u00e7\u00f6z\u00fcnebilir<\/td>\n<\/tr>\n
\u00e7\u00f6z\u00fcn\u00fcrl\u00fck<\/td>\n Etanol, DMSO ve DMF’de \u00e7\u00f6z\u00fcn\u00fcr<\/td>\n<\/tr>\n
Buhar bas\u0131nc\u0131<\/td>\n Uygulanamaz<\/td>\n<\/tr>\n
Buhar yo\u011funlu\u011fu<\/td>\n Uygulanamaz<\/td>\n<\/tr>\n
pKa<\/td>\n 4.0\u20135.6<\/td>\n<\/tr>\n
pH<\/td>\n 3,0 (sar\u0131)\u20134,6 (mavi)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n

Bromofenol mavisinin g\u00fcvenli\u011fi ve tehlikeleri<\/strong><\/h5>\n

BPB do\u011fru kullan\u0131ld\u0131\u011f\u0131nda nispeten g\u00fcvenli bir bile\u015fiktir. Kanserojen, mutajenik veya teratojenik olarak s\u0131n\u0131fland\u0131r\u0131lmam\u0131\u015ft\u0131r. Ancak t\u00fcm kimyasallarda oldu\u011fu gibi BPB’yi kullan\u0131rken de uygun \u00f6nlemlerin al\u0131nmas\u0131 \u00f6nemlidir. Bu, temas halinde ciltte ve g\u00f6zde tahri\u015fe neden olabilir. Yutma bulant\u0131 ve kusmaya neden olabilir. Cilt ile temas\u0131 halinde bol sabun ve su ile y\u0131kay\u0131n\u0131z. G\u00f6z ile temas\u0131 halinde bol su ile birka\u00e7 dakika y\u0131kay\u0131n. BPB’yi kullan\u0131rken toz veya buharlar\u0131n solunmas\u0131ndan ka\u00e7\u0131nmak ve eldiven ve g\u00f6zl\u00fck gibi uygun koruyucu ekipmanlar\u0131n kullan\u0131lmas\u0131 da \u00f6nemlidir.<\/p>\n

\n\n\n\n\n\n\n\n\n\n\n
Tehlike sembolleri<\/td>\n Hi\u00e7biri<\/td>\n<\/tr>\n
G\u00fcvenlik A\u00e7\u0131klamas\u0131<\/td>\n S24\/25 \u2013 Cilt ve g\u00f6zlerle temas\u0131ndan ka\u00e7\u0131n\u0131n<\/td>\n<\/tr>\n
<\/td>\n S36\/37\/39 \u2013 Uygun koruyucu k\u0131yafet, eldiven ve g\u00f6zl\u00fck kullan\u0131n<\/td>\n<\/tr>\n
BM kimlik numaralar\u0131<\/td>\n D\u00fczenlenmemi\u015f<\/td>\n<\/tr>\n
HS kodu<\/td>\n 2934.99.9000<\/td>\n<\/tr>\n
Tehlike s\u0131n\u0131f\u0131<\/td>\n s\u0131n\u0131fland\u0131r\u0131lmam\u0131\u015f<\/td>\n<\/tr>\n
Paketleme grubu<\/td>\n Uygulanamaz<\/td>\n<\/tr>\n
Toksisite<\/td>\n LD50 (oral, s\u0131\u00e7an): > 5.000 mg\/kg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n
Bromofenol mavisinin sentezi i\u00e7in y\u00f6ntemler<\/strong><\/h5>\n

Bromofenol mavisini sentezlemek i\u00e7in \u00e7e\u015fitli y\u00f6ntemler vard\u0131r.<\/p>\n

Yayg\u0131n bir y\u00f6ntem, sodyum hidroksit varl\u0131\u011f\u0131nda 3,5-dibromo-4-hidroksibenzaldehid ile 4-aminofenol aras\u0131ndaki reaksiyondur. Bu reaksiyon, mavi-ye\u015fil bir kat\u0131 formunda bromofenol mavisinin olu\u015fumuna yol a\u00e7ar. \u00dcr\u00fcn su veya etanolden yeniden kristalle\u015ftirme yoluyla safla\u015ft\u0131r\u0131labilir.<\/p>\n

Ba\u015fka bir y\u00f6ntem, asetik anhidrit ve s\u00fclf\u00fcrik asit varl\u0131\u011f\u0131nda 4-dimetilaminobenzaldehit ile 3,5-dibromosalisilik asit aras\u0131ndaki reaksiyonu i\u00e7erir. Bu reaksiyon, etanolden yeniden kristalle\u015ftirme yoluyla safla\u015ft\u0131r\u0131labilen mavi bir kat\u0131 halinde bromofenol mavisini verir.<\/p>\n

Ba\u015fka bir y\u00f6ntem, sodyum hidroksit varl\u0131\u011f\u0131nda 3,5-dibromo-4-hidroksibenzoik asit ile 4-aminofenol aras\u0131ndaki reaksiyonu i\u00e7erir. Bu reaksiyon, su veya etanolden yeniden kristalle\u015ftirme yoluyla safla\u015ft\u0131r\u0131labilen mavi-ye\u015fil bir kat\u0131n\u0131n olu\u015fumuna yol a\u00e7ar.<\/p>\n

Bromofenol mavisinin kullan\u0131m alanlar\u0131<\/strong><\/h5>\n

Bromofenol mavisi benzersiz \u00f6zelliklerinden dolay\u0131 farkl\u0131 alanlarda bir\u00e7ok kullan\u0131ma sahiptir. Bromofenol mavisinin ana kullan\u0131mlar\u0131ndan baz\u0131lar\u0131 \u015funlard\u0131r:<\/p>\n