{"id":557,"date":"2023-07-22T14:18:20","date_gmt":"2023-07-22T14:18:20","guid":{"rendered":"https:\/\/chemuza.org\/cn\/%e6%ba%b4%e9%85%9a%e8%93%9dc19h10br4o5s\/"},"modified":"2023-07-22T14:18:20","modified_gmt":"2023-07-22T14:18:20","slug":"%e6%ba%b4%e9%85%9a%e8%93%9dc19h10br4o5s","status":"publish","type":"post","link":"https:\/\/chemuza.org\/cn\/%e6%ba%b4%e9%85%9a%e8%93%9dc19h10br4o5s\/","title":{"rendered":"\u6eb4\u915a\u84dd \u2013 c19h10br4o5s, 115-39-9"},"content":{"rendered":"<p>\u6eb4\u915a\u84dd (BPB) \u662f\u4e00\u79cd pH \u6307\u793a\u5242\uff0c\u5728 pH \u503c\u4f4e\u4e8e 3.0 \u65f6\u53d8\u9ec4\uff0c\u5728 pH \u503c\u9ad8\u4e8e 4.6 \u65f6\u53d8\u84dd\u3002\u5b83\u5728\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u901a\u5e38\u7528\u4f5c DNA \u7535\u6cf3\u7684\u793a\u8e2a\u67d3\u6599\u3002<\/p>\n<figure class=\"wp-block-table\">\n<table>\n<tbody>\n<tr>\n<td>\u56fd\u9645\u7eaf\u7cb9\u548c\u5e94\u7528\u5316\u5b66\u8054\u5408\u4f1a\u540d\u79f0<\/td>\n<td>3\u2032,3\u2033,5\u2032,5\u2033-\u56db\u6eb4\u82ef\u915a\u78fa\u915e<\/td>\n<\/tr>\n<tr>\n<td>\u5206\u5b50\u5f0f<\/td>\n<td>C19H10Br4O5S<\/td>\n<\/tr>\n<tr>\n<td> CAS \u53f7<\/td>\n<td>115-39-9<\/td>\n<\/tr>\n<tr>\n<td>\u540c\u4e49\u8bcd<\/td>\n<td>3\u2032,3\u2033,5\u2032,5\u2033-\u56db\u6eb4\u915a\u78fa\u915e\u3001TBB\u3001\u6eb4\u915a\u84dd\u94a0\u76d0\u548c\u6eb4\u915a\u84dd\u6e38\u79bb\u9178<\/td>\n<\/tr>\n<tr>\n<td>\u4e59\u9170\u80c6\u78b1<\/td>\n<td>InChI=1S\/C19H10Br4O5S\/c20-13-5-1-12(2-6-13)19(11-24)27(25,26)17-9-15(29(21,22)23)7- 3-14(17)18(10-16(19)30(25,26)28)31(4-8-32(25,26)27)33(34)35\/h1-10,24H,11H2, (H,21,22,23)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<p>\u6eb4\u915a\u84dd \u6469\u5c14\u8d28\u91cf<\/p>\n<p>BPB \u7684\u6469\u5c14\u8d28\u91cf\u4e3a 669.96 g\/mol\u3002\u6469\u5c14\u8d28\u91cf\u662f\u6307\u4e00\u6469\u5c14\u7269\u8d28\u7684\u8d28\u91cf\uff0c\u4ee5\u514b\u6bcf\u6469\u5c14\u8868\u793a\u3002\u5316\u5408\u7269\u7684\u6469\u5c14\u8d28\u91cf\u53ef\u4ee5\u901a\u8fc7\u5c06\u5176\u6bcf\u4e2a\u7ec4\u6210\u539f\u5b50\u7684\u539f\u5b50\u8d28\u91cf\u76f8\u52a0\u6765\u8ba1\u7b97\u3002 BPB\u662f\u4e00\u79cd\u590d\u6742\u7684\u6709\u673a\u5316\u5408\u7269\uff0c\u542b\u6709\u78b3\u3001\u6c22\u3001\u6c27\u3001\u786b\u548c\u6eb4\u539f\u5b50\uff0c\u5176\u6469\u5c14\u8d28\u91cf\u662f\u901a\u8fc7\u5c06\u5316\u5408\u7269\u4e2d\u6bcf\u79cd\u5143\u7d20\u7684\u539f\u5b50\u8d28\u91cf\u76f8\u52a0\u6765\u8ba1\u7b97\u7684\u3002<\/p>\n<h6 class=\"wp-block-heading\">\u6eb4\u915a\u84dd\u7684\u6cb8\u70b9<\/h6>\n<p>BPB \u7684\u6cb8\u70b9\u7ea6\u4e3a 600\u00b0C\u3002\u7269\u8d28\u7684\u6cb8\u70b9\u662f\u8be5\u7269\u8d28\u7684\u84b8\u6c14\u538b\u7b49\u4e8e\u5927\u6c14\u538b\u529b\u65f6\u7684\u6e29\u5ea6\u3002\u5728\u6b64\u6e29\u5ea6\u4e0b\uff0c\u6db2\u4f53\u8f6c\u53d8\u4e3a\u6c14\u6001\u3002 BPB \u7531\u4e8e\u5c3a\u5bf8\u5927\u4e14\u7ed3\u6784\u590d\u6742\u800c\u5177\u6709\u9ad8\u6cb8\u70b9\uff0c\u9700\u8981\u5927\u91cf\u80fd\u91cf\u6765\u5206\u89e3\u5c06\u5206\u5b50\u7ed3\u5408\u5728\u4e00\u8d77\u7684\u5206\u5b50\u95f4\u529b\u3002<\/p>\n<p>\u6eb4\u915a\u84dd\u7194\u70b9<\/p>\n<p>BPB \u7684\u7194\u70b9\u7ea6\u4e3a 218\u00b0C\u3002\u7269\u8d28\u7684\u7194\u70b9\u662f\u7269\u8d28\u7684\u56fa\u6001\u8f6c\u53d8\u4e3a\u6db2\u6001\u7684\u6e29\u5ea6\u3002\u7531\u4e8e\u6676\u683c\u4e2d\u5b58\u5728\u8f83\u5f3a\u7684\u5206\u5b50\u95f4\u529b\uff0cBPB \u7684\u7194\u70b9\u76f8\u5bf9\u8f83\u9ad8\u3002<\/p>\n<h6 class=\"wp-block-heading\">\u6eb4\u915a\u84dd \u5bc6\u5ea6 g\/ml<\/h6>\n<p> BPB \u7684\u5bc6\u5ea6\u7ea6\u4e3a 1.8 g\/ml\u3002\u5bc6\u5ea6\u662f\u63cf\u8ff0\u7269\u8d28\u6bcf\u5355\u4f4d\u4f53\u79ef\u7684\u8d28\u91cf\u7684\u7269\u7406\u6027\u8d28\u3002\u7531\u4e8e\u5176\u7ed3\u6784\u590d\u6742\uff0cBPB\u7684\u5bc6\u5ea6\u76f8\u5bf9\u8f83\u9ad8\uff0c\u5bfc\u81f4\u5355\u4f4d\u4f53\u79ef\u8d28\u91cf\u8f83\u9ad8\u3002<\/p>\n<p>\u6eb4\u915a\u84dd\u5206\u5b50\u91cf<\/p>\n<p>BPB\u7684\u5206\u5b50\u91cf\u4e3a669.96 g\/mol\u3002\u5206\u5b50\u91cf\u662f\u5206\u5b50\u4e2d\u6240\u6709\u539f\u5b50\u7684\u539f\u5b50\u91cf\u4e4b\u548c\u3002\u7269\u8d28\u7684\u5206\u5b50\u91cf\u7528\u4e8e\u5404\u79cd\u8ba1\u7b97\uff0c\u4f8b\u5982\u786e\u5b9a\u5236\u5907\u7ed9\u5b9a\u6d53\u5ea6\u7684\u6eb6\u6db2\u6240\u9700\u7684\u7269\u8d28\u7684\u91cf\u3002 <\/p>\n<div class=\"wp-block-image\">\n<figure class=\"alignright size-large is-resized\"><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/chemuza.org\/wp-content\/uploads\/2023\/08\/BPB.jpg\" alt=\"\u6eb4\u915a\u84dd\" width=\"160\" height=\"165\" srcset=\"\" sizes=\"auto, \"><\/figure>\n<\/div>\n<p>\u6eb4\u915a\u84dd\u7684\u7ed3\u6784\u5f0f<\/p>\n<p>BPB \u5177\u6709\u78fa\u915e\u7ed3\u6784\uff0c\u542b\u6709\u56db\u4e2a\u6eb4\u539f\u5b50\u3001\u4e24\u4e2a\u915a\u57fa\u56e2\u548c\u4e00\u4e2a\u78fa\u9178\u57fa\u56e2\u3002\u8be5\u5206\u5b50\u5177\u6709\u4e00\u4e2a\u4e0e\u4e24\u4e2a\u915a\u57fa\u56e2\u548c\u4e00\u4e2a\u78fa\u9178\u57fa\u56e2\u8fde\u63a5\u7684\u4e2d\u5fc3\u78b3\u539f\u5b50\u3002\u8be5\u5206\u5b50\u8fd8\u542b\u6709\u4e24\u4e2a\u8fde\u63a5\u5230\u6bcf\u4e2a\u82ef\u915a\u57fa\u56e2\u7684\u6eb4\u539f\u5b50\u3002\u78fa\u9178\u57fa\u5e26\u8d1f\u7535\uff0c\u4f7f BPB \u6210\u4e3a\u6c34\u6eb6\u6027\u67d3\u6599\u3002<\/p>\n<h6 class=\"wp-block-heading\">\u6eb4\u915a\u84dd\u914d\u65b9<\/h6>\n<p>BPB\u7684\u5316\u5b66\u5f0f\u4e3aC19H10Br4O5S\u3002\u8be5\u5f0f\u8868\u793a\u5206\u5b50\u7684\u7ec4\u6210\uff0c\u4ee5\u5b58\u5728\u7684\u539f\u5b50\u7684\u6570\u91cf\u548c\u7c7b\u578b\u8868\u793a\u3002\u5f0f\u4e2d\u8868\u660eBPB\u542b\u670919\u4e2a\u78b3\u539f\u5b50\u300110\u4e2a\u6c22\u539f\u5b50\u30014\u4e2a\u6eb4\u539f\u5b50\u30015\u4e2a\u6c27\u539f\u5b50\u548c1\u4e2a\u786b\u539f\u5b50\u3002<\/p>\n<figure class=\"wp-block-table\">\n<table>\n<tbody>\n<tr>\n<td>\u5916\u8c8c<\/td>\n<td>\u6df1\u84dd\u8272\u6676\u4f53\u6216\u7c89\u672b<\/td>\n<\/tr>\n<tr>\n<td>\u6bd4\u91cd<\/td>\n<td>1.82 \u81f3 1.85 \u514b\/\u7acb\u65b9\u5398\u7c73<\/td>\n<\/tr>\n<tr>\n<td>\u989c\u8272<\/td>\n<td>\u6df1\u84dd<\/td>\n<\/tr>\n<tr>\n<td>\u95fb<\/td>\n<td>\u65e0\u5473<\/td>\n<\/tr>\n<tr>\n<td>\u6469\u5c14\u8d28\u91cf<\/td>\n<td>669.96 \u514b\/\u6469\u5c14<\/td>\n<\/tr>\n<tr>\n<td>\u5bc6\u5ea6<\/td>\n<td>1.8\u514b\/\u7acb\u65b9\u5398\u7c73<\/td>\n<\/tr>\n<tr>\n<td>\u878d\u5408\u70b9<\/td>\n<td>\u301c218\u00b0C<\/td>\n<\/tr>\n<tr>\n<td>\u6cb8\u70b9<\/td>\n<td>\u301c600\u00b0C<\/td>\n<\/tr>\n<tr>\n<td>\u95ea\u70b9<\/td>\n<td>\u4e0d\u9002\u7528<\/td>\n<\/tr>\n<tr>\n<td>\u6c34\u4e2d\u6eb6\u89e3\u5ea6<\/td>\n<td>\u6eb6\u4e8e\u6c34<\/td>\n<\/tr>\n<tr>\n<td>\u6eb6\u89e3\u5ea6<\/td>\n<td>\u6eb6\u4e8e\u4e59\u9187\u3001DMSO \u548c DMF<\/td>\n<\/tr>\n<tr>\n<td>\u84b8\u6c7d\u538b\u529b<\/td>\n<td>\u4e0d\u9002\u7528<\/td>\n<\/tr>\n<tr>\n<td>\u84b8\u6c14\u5bc6\u5ea6<\/td>\n<td>\u4e0d\u9002\u7528<\/td>\n<\/tr>\n<tr>\n<td>\u9178\u5ea6<\/td>\n<td>4.0\u20135.6<\/td>\n<\/tr>\n<tr>\n<td>\u9178\u78b1\u5ea6<\/td>\n<td>3.0\uff08\u9ec4\u8272\uff09\u20134.6\uff08\u84dd\u8272\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<h5 class=\"wp-block-heading\"><strong><br \/>\u6eb4\u915a\u84dd\u7684\u5b89\u5168\u6027\u548c\u5371\u9669\u6027<\/strong><\/h5>\n<p>\u5982\u679c\u5904\u7406\u5f97\u5f53\uff0cBPB \u662f\u4e00\u79cd\u76f8\u5bf9\u5b89\u5168\u7684\u5316\u5408\u7269\u3002\u5b83\u4e0d\u5c5e\u4e8e\u81f4\u764c\u3001\u81f4\u7a81\u53d8\u6216\u81f4\u7578\u7269\u8d28\u3002\u7136\u800c\uff0c\u4e0e\u6240\u6709\u5316\u5b66\u54c1\u4e00\u6837\uff0c\u5728\u5904\u7406 BPB \u65f6\u91c7\u53d6\u9002\u5f53\u7684\u9884\u9632\u63aa\u65bd\u975e\u5e38\u91cd\u8981\u3002\u63a5\u89e6\u540e\u53ef\u80fd\u4f1a\u523a\u6fc0\u76ae\u80a4\u548c\u773c\u775b\u3002\u98df\u5165\u53ef\u80fd\u4f1a\u5f15\u8d77\u6076\u5fc3\u548c\u5455\u5410\u3002\u5982\u679c\u63a5\u89e6\u76ae\u80a4\uff0c\u8bf7\u7528\u5927\u91cf\u80a5\u7682\u548c\u6c34\u6e05\u6d17\u3002\u5982\u679c\u63a5\u89e6\u773c\u775b\uff0c\u8bf7\u7528\u5927\u91cf\u6c34\u51b2\u6d17\u51e0\u5206\u949f\u3002\u5728\u5904\u7406 BPB \u65f6\uff0c\u907f\u514d\u5438\u5165\u7070\u5c18\u6216\u84b8\u6c7d\u5e76\u4f7f\u7528\u9002\u5f53\u7684\u9632\u62a4\u8bbe\u5907\uff08\u4f8b\u5982\u624b\u5957\u548c\u62a4\u76ee\u955c\uff09\u4e5f\u5f88\u91cd\u8981\u3002<\/p>\n<figure class=\"wp-block-table\">\n<table>\n<tbody>\n<tr>\n<td>\u5371\u9669\u7b26\u53f7<\/td>\n<td>\u6ca1\u6709\u4efb\u4f55<\/td>\n<\/tr>\n<tr>\n<td>\u5b89\u5168\u8bf4\u660e<\/td>\n<td>S24\/25 \u2013 \u907f\u514d\u63a5\u89e6\u76ae\u80a4\u548c\u773c\u775b<\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td>S36\/37\/39 \u2013 \u7a7f\u6234\u9002\u5f53\u7684\u9632\u62a4\u670d\u3001\u624b\u5957\u548c\u62a4\u76ee\u955c<\/td>\n<\/tr>\n<tr>\n<td>\u8054\u5408\u56fd\u8bc6\u522b\u53f7<\/td>\n<td>\u4e0d\u89c4\u8303<\/td>\n<\/tr>\n<tr>\n<td>\u6d77\u5173\u7f16\u7801<\/td>\n<td>2934.99.9000<\/td>\n<\/tr>\n<tr>\n<td>\u5371\u9669\u7b49\u7ea7<\/td>\n<td>\u672a\u5206\u7c7b<\/td>\n<\/tr>\n<tr>\n<td>\u5305\u88c5\u7ec4\u522b<\/td>\n<td>\u4e0d\u9002\u7528<\/td>\n<\/tr>\n<tr>\n<td>\u6bd2\u6027<\/td>\n<td>LD50\uff08\u5927\u9f20\u53e3\u670d\uff09\uff1a&gt; 5,000 mg\/kg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<h5 class=\"wp-block-heading\"><strong>\u6eb4\u915a\u84dd\u7684\u5408\u6210\u65b9\u6cd5<\/strong><\/h5>\n<p>\u6eb4\u915a\u84dd\u7684\u5408\u6210\u65b9\u6cd5\u6709\u591a\u79cd\u3002<\/p>\n<p>\u5e38\u89c1\u7684\u65b9\u6cd5\u662f3,5-\u4e8c\u6eb4-4-\u7f9f\u57fa\u82ef\u7532\u919b\u548c4-\u6c28\u57fa\u82ef\u915a\u5728\u6c22\u6c27\u5316\u94a0\u5b58\u5728\u4e0b\u53cd\u5e94\u3002\u8be5\u53cd\u5e94\u5bfc\u81f4\u5f62\u6210\u84dd\u7eff\u8272\u56fa\u4f53\u5f62\u5f0f\u7684\u6eb4\u915a\u84dd\u3002\u4ea7\u7269\u53ef\u4ee5\u901a\u8fc7\u4ece\u6c34\u6216\u4e59\u9187\u4e2d\u91cd\u7ed3\u6676\u6765\u7eaf\u5316\u3002<\/p>\n<p>\u53e6\u4e00\u79cd\u65b9\u6cd5\u6d89\u53ca4-\u4e8c\u7532\u6c28\u57fa\u82ef\u7532\u919b\u548c3,5-\u4e8c\u6eb4\u6c34\u6768\u9178\u5728\u4e59\u9178\u9150\u548c\u786b\u9178\u5b58\u5728\u4e0b\u7684\u53cd\u5e94\u3002\u8be5\u53cd\u5e94\u4ea7\u751f\u84dd\u8272\u56fa\u4f53\u72b6\u7684\u6eb4\u915a\u84dd\uff0c\u53ef\u4ee5\u901a\u8fc7\u4ece\u4e59\u9187\u4e2d\u91cd\u7ed3\u6676\u6765\u7eaf\u5316\u3002<\/p>\n<p>\u53e6\u4e00\u79cd\u65b9\u6cd5\u6d89\u53ca3,5-\u4e8c\u6eb4-4-\u7f9f\u57fa\u82ef\u7532\u9178\u548c4-\u6c28\u57fa\u82ef\u915a\u5728\u6c22\u6c27\u5316\u94a0\u5b58\u5728\u4e0b\u7684\u53cd\u5e94\u3002\u8be5\u53cd\u5e94\u5bfc\u81f4\u5f62\u6210\u84dd\u7eff\u8272\u56fa\u4f53\uff0c\u53ef\u4ee5\u901a\u8fc7\u4ece\u6c34\u6216\u4e59\u9187\u4e2d\u91cd\u7ed3\u6676\u6765\u7eaf\u5316\u3002<\/p>\n<h5 class=\"wp-block-heading\"><strong>\u6eb4\u915a\u84dd\u7684\u7528\u9014<\/strong><\/h5>\n<p>\u6eb4\u915a\u84dd\u7531\u4e8e\u5176\u72ec\u7279\u7684\u6027\u8d28\uff0c\u5728\u4e0d\u540c\u9886\u57df\u5177\u6709\u591a\u79cd\u7528\u9014\u3002\u4ee5\u4e0b\u662f\u6eb4\u915a\u84dd\u7684\u4e00\u4e9b\u4e3b\u8981\u7528\u9014\uff1a<\/p>\n<ul>\n<li>\u751f\u7269\u67d3\u8272\uff1a\u5728\u7535\u6cf3\u5e94\u7528\u4e2d\u7528\u4f5c\u751f\u7269\u67d3\u6599\u3002\u7528\u4f5c\u8ffd\u8e2a\u67d3\u6599\uff0c\u76d1\u6d4b\u51dd\u80f6\u7535\u6cf3\u8fc7\u7a0b\u4e2d\u86cb\u767d\u8d28\u6216\u6838\u9178\u8fc1\u79fb\u7684\u8fdb\u5ea6\u3002<\/li>\n<li> pH\u6307\u793a\u5242\uff1a\u7528\u4f5cpH\u6307\u793a\u5242\uff0c\u8fc7\u6e21\u8303\u56f4\u5728pH 3.0\u81f34.6\u4e4b\u95f4\u3002\u968f\u7740\u6eb6\u6db2 pH 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